Release of Several Enzymes from Rat Liver Microsomes by Various Solubilizing Treatments

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Rat linver microsomes were solubilized by treatments of detergents, hydrolyzing enzymes, sonication and freezing-thawing, and the effects of these solubilizing treatments On the release of four enzymes, NADH-cytochrome b5 reductase (Fp1), NADPH-cytochrome c reductase (Fp2), γ-glutamyltranspeptidase (γ-GTP) and glucose-6-phosphatase (G-6-Pase) from the microsomes were investigated. Fp1, Fp2 and γ-GTP were releared easily by Triton X-100, a nonionic detergent.Fp1 and Fp2 were releared much more than γ-GTP by cholic acid, an anionic detergent.Trypsin or lipase digestion caused only the release of Fp2 from the microsomes.ln the case of sonication treatment, the release of each enzyme with an increase in treatment time was not observed.These enzymes were released only a little by twenty times of freezing-thawing. The present results suggest that Fp2 might be most released from the membrane of damaged linver microsomes.