Effects of Aging and Sex Diffrence on Superoxide Dismutase Content in Human Red Blood Cell

概要

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This study was designed to evaluate changes in the supenoxide dismutase (SOD) quantity in human erythrocytes based on aging and sex differences.<BR>The recovery rate of SOD quantiy in the supernatant after removing hemoglobin with organic solvent (ethylalcohol: CHCl<SUB>3</SUB>=5: 3) from whole blood was 96%.SOD activity in whole blood was stable for at least 6 days in refrigerator and SOD activity in the supernatant aften removing hemoglobin was not changed for 5 hours at room temperature.<BR>Both SOD quantity in whole blood of nonmal human subjects and its variations with regand to sex and age were detected.The SOD quantity per 1 ml of whole blood was69.5±15.1μg in males and62.8±11.5μg in females, a significantly lower value in females. However, SOD quantity per hemoglobin (g) was 512.7±104.2μg in males and508.9±93.2μg in females.The SOD quantity per1010RBC was154.9±44.5μg in males and152.6±28.1μg in females.There was a tendency for the SOD quantity in whole blood to decreasewith aging, However, no changes secondary to aging was noted in relation to hemoglobin (g) and erythrocytes.
Japan Society of Clinical Chemistryの論文