The detection and production of nitric oxide in synovial fluids from patients with acute gouty arthritis

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Nitric oxide (NO) is a potent mediator of physiological functions and pathogenic reactions in human disease. Recently, considable evidence supporting the pathogenic mechanism of NO in some arthritides has been demonstrated. We investigated the role of NO in the pathogenesis of acute gouty arthritis. Synovial fluids (SF) from gouty arthritis contained a high level of NO, compared to SF from osteoar thritis, rheumatoid arthritis (RA) and palindr omic rheumatism patients. Monocytes from normal peripheral blood did not produce NO, synthetic monosodium urate (MSU) crystals. However, activated macrophage(Mφ)in SF from active RA patients produced a high level of NO after stimulation with fresh serum-treated MSU crystals. These NO productions were augmented by the addition of inflammatory cytokines (interleukin-1 β or tumor necrosis factor α). Presenceof a specific antagonist of NO synthetase (NGmonomethyl-L-arginine) effectively inhibited the production of NO from Mφ. From these findings NO would be a potent mediator of acute gouty arthritis. after stimulation with fresh serum-treated
Japanese Society of Gout and Nucleic Acid Metabolismの論文