尿中T3の測定

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Triiodothyronine in urine was measured by direct radioimmunoassay. For the standard curve, 100μl of antiserum (1 : 40,000) was incubated with 125I-T3, and varying amounts of unlabeled L-T3 were added. The cross-reaction of L-T3 with L-T4 in this assay system was less than 0.2 per cent. Dilution of high T3 urine was paralleled to the standard curve. Various amounts of. L-T3 (50 to 400 μg/ 100 ml) were added to urine and recovery was 101 8 per cent (mean±s.d.). Coefficients of variation were 3-8 per cent within each assay and 13 per cent between assays. Among 45 euthyroid sick patients, urinary T3 levels were 0.3 to 1.96 μg per 24 hrs (mean ± s.d., 0.81 ± 0.39 per 24 hrs). Among 18 hyperthyroid patients, urinary T3 levels were 2.9 to 14.6 μg per 24 hrs (mean ± s.d., 7.48 ± 3.32 μg per 24 hrs). Among 14 hypothyroid patients, urinary T3 levels were 0 to 0.54 μg per 24 hrs (mean ± s.d., 0.14 ± 0.15 μg per 24 hrs). Among 11 pregnant women, urinary T3 levels were 0.58 to 1.76μg per 24 hrs (mean ± s.d., 1.06 ± 0.39μg per 24 hrs) and they were all within normal range. Among 6 uremic patients, urinary T3 levels were less than 0.1 μg per 24 hrs. Positive correlations were found between urinary T3 and serum total T3 (r=0.89) and serum free T3 (r=0.97). A positive correlation was also found between urinary T3 and creatinine clearance (r=0.63). Mean urinary clearance of T3 (CT3=Urinary T3 excretion/Serum free T3) was 166 ml/min (174 mi/min in euthyroid sick, 166 ml/min in hyperthyroidism, and 106 in hypothyroidism) and it was higher as compared to their mean creatinine clearance of 110 ml/ min. The measurement of T3 in urine is reliable and easy to perform, and may allow a new approach to the understanding of thyroid hormone metabolism.
日本内分泌学会の論文

尿中T<SUP>3</SUP>の測定

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