先天性副腎皮質過形成 (21-水酸化酵素欠損症) のマス・スクリーニングに関する研究:第2報-高速液体クロマトグラフィーによる血中および羊水中ステロイドの一斉分析-

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A quantitative analysis of steroids in serum, dried blood samples on filter paper and amniotic fluid with high-performance liquid chromatography has been developed and applied to the diagnosis of risk infants on neonatal screening for congenital adrenal hyperplasia. The present method is simple, rapid and accurate. The detection limits of cortisol and 17a-hydroxyprogesterone are 0.2 ng and 0.3 ng, respectively. The twenty-two steroids can be analysed within 60 minutes using the isogradient mobile phase. The method is highly correlated with radioimmunoassay and enzymeimmunoassay.<BR>In 4 patients with the salt-losing form, 17a-hydroxyprogesterone was higher and cortisol lower than in 4 pateints with the simple-virilizing form.<BR>This method seems suitable for the routine clinical elucidation of congenital adrenal hyperplasia.