The Effects of 2-Aminoethoxydiphenyl Borate and Diphenylboronic Anhydride on Gap Junctions Composed of Connexin43 in TM4 Sertoli Cells
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概要
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2-Aminoethoxydiphenyl borate (2-APB) has recently been demonstrated to inhibit gap junction (GJ) channels, whereas the underlying mechanisms are still unknown. Using mouse TM4 Sertoli cell which expresses connexin43 (Cx43), we explored the effects of 2-APB and its analogues on dye-coupling through junctional channels formed by Cx43 and on expression of Cx43. Exposure of the cells to 2-APB (1—50 μM) and one of its analogues diphenylboronic anhydride (DPBA) (1—30 μM) for 4 h leads to a significant decrease in dye coupling of GJ in a concentration-dependent manner. The inhibitory effects of 2-APB and DPBA are reversible since decreased GJ coupling resumes after the two compounds are washed out. The disfunction of GJ induced by 2-APB and DPBA is associated with a decrease in total amount of Cx43 protein and number of GJs on the cell membrane. 2-APB and DPBA do not alter Cx43 phosphorylation state and the level of Cx43 mRNA expression. The loss of Cx43 protein is prevented by either lysosomal or proteasomal inhibitor, suggesting that the decrease in Cx43 results from a 2-APB or DPBA-enhanced degradation of Cx43. The present results indicate that 2-APB and DPBA inhibit GJ communication through decreasing Cx43 expression in TM4 cells.
著者
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Tao Liang
Department Of Electrical Electronics And Computer Engineering. Faculty Of Engineering Chiba Institut
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Wang Qin
Department Of Chemistry Shanghai Normal University
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Yang Yan
Department Of Chemical Engineering Anhui University Of Science And Technology
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Cao Ming-hui
Department Of Anesthesia The Second Affiliated Hospital Sun Yat-sen University
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Li Li
Department Of Cancer Epidemiology Chinese Academy Of Medical Sciences
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Yuan Dong-dong
Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University
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Cao Ming-hui
Department of Anaesthesia, The Second Affiliated Hospital, Sun Yat-Sen University
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Wang Qin
Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University
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Yang Yan
Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University
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