In Vitro and in Vivo Antimicrobial Effect of Photodynamic Therapy Using a Highly Pure Chlorin e6 against Staphylococcus aureus Xen29
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概要
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Photodynamic therapy (PDT) has been recommended as an alternative therapy for various diseases including microbial infection. Recently, we developed a new method for the preparation of highly pure chlorin e6 (Ce6), which has been widely used as a second-generation photosensitizer. PDT using Ce6 was very effective for inhibition of in vitro growth of several bacterial strains. To clarify a possibility for its clinical application, in this study, we examined in vitro and in vivo antimicrobial effects of Ce6-mediated PDT in mice model of skin infection of Staphylococcus aureus Xen29. Inhibition zone analysis and colony forming unit (CFU) count revealed that Ce6-mediated PDT inhibited effectively in vitro bacterial growth. In addition, biofilm formation ability of S. aureus Xen29 was decreased by Ce6-mediated PDT. In vivo experiment, mice receiving Ce6-mediated PDT exhibited less intensity of bioluminescent signal, showing significant inhibition of bacterial growth. Furthermore, in histopathological examination, marked neutrophilic infiltration and massive bacterial colonies were seen in control mice and mice receiving laser or Ce6 alone, but not in mice treated with PDT. These results suggest that PDT using Ce6 extracted by our new method can be clinically useful against bacterial infectious diseases.
著者
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PARK Jong-Hwan
Department of Laboratory Animal Medicine
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Kim Yong-chul
Department Of Life Science Gwangju Institute Of Science And Technology
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Kim Soo-A
Department of Biochemistry, College of Oriental Medicine, Dongguk University
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Ahn Sang-Gun
Department of Pathology, School of Dentistry, Chosun University
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Yoon Jung-Hoon
Department of Pathology, School of Dentistry, Chosun University
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Ahn Mee-Young
Department of Pathology, Research Center for Oral Disease Regulation of the Aged, School of Dentistry, Chosun University
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Moon Yeon-Hee
Department of Dental Hygiene, Chodang University
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Yoon Jung-Hoon
Department of Oral & Maxillofacial Pathology, College of Dentistry, Daejeon Dental Hospital, Wonkwang University
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Ahn Sang-Gun
Department of Pathology, Research Center for Oral Disease Regulation of the Aged, School of Dentistry, Chosun University
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KIM Yong-Chul
Department of Electrical Engineering, Pusan National University Jangjeon-dong
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Park Jong-Hwan
Department of Biochemistry, College of Medicine, Konyang University
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