Development of a Single Bovine Embryo Improved by Co-culture with Trophoblastic Vesicles in Vitamin-supplemented Medium
スポンサーリンク
概要
- 論文の詳細を見る
To improve the development of singly cultured bovine embryos, we developed a co-culture method with trophoblastic vesicles. The growth of trophoblastic cells was markedly increased in vitamin-supplemented medium 199 compared with medium 199. Upon co-culture of a single embryo with trophoblastic vesicles in vitamin-supplemented medium 199, embryo development to the blastocyst stage was significantly higher than in embryos co-cultured with trophoblastic vesicles in RPMI 1640 or with cumulus cells in medium 199 (control). In the absence of the vitamin cocktail, co-culture with trophoblastic vesicles in medium 199 did not improve embryo development compared with that of the control. The vitamin cocktail was effective in embryo development when co-cultured with trophoblastic vesicles, but not with cumulus cells. Embryo development was not improved in the absence of co-cultured trophoblastic vesicles, even in the presence of vitamin cocktail. In conclusion, the co-culture system with trophoblastic vesicles in vitamin-supplemented medium 199 efficiently enhances the development of singly cultured embryos.
著者
-
Hattori Masa-aki
Department Of Animal And Marine Bioresource Sciences Faculty Of Agriculture Graduate School Kyushu U
-
UEDA Shuji
Department of Agriculture, Forestry and Fisheries, Fukuoka Prefectural Government, Fukuoka 812-8577, Japan
-
MORI Miyuki
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
-
HATTORI Masa-aki
Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture, Kyushu University, Fukuoka 812-8581, Japan
-
KASA Shojiro
Fukuoka Agricultural Research Center, Fukuoka 818-8549, Japan
関連論文
- The Modified High-Density Survival Assay is the Useful Tool to Predict the Effectiveness of Fractionated Radiation Exposure
- Upregulation of P-Glycoprotein Activity in Porcine Oocytes and Granulosa Cells During In Vitro Maturation
- Nitric oxide and ovarian function
- Growth or Differentiation: Determintaion by FSH of the Action of Insulin-Like Growth Factor-I in Cultured Rat Granulosa Cells
- Chicken Dead End Homologue Protein is a Nucleoprotein of Germ Cells Including Primordial Germ Cells
- Expression of Hedgehog Family Genes in the Rat Uterus During Early Pregnancy
- Constitutively Activated Rho Gunosine Triphosphatases Regulate the Growth and Morphology of Hairy Cell Leukemia Cells
- Constitutive Activation of c-kit by the Juxtamembrane but Not the Catalytic Domain Mutations Is Inhibited Selectively by Tyrosine Kinase Inhibitors STI571 and AG1296
- KIT MUTATIONS IN HEMATOPOIETIC MALIGNANCIES
- EFFECTS OF ENALAPRIL ON LEFT VENTRICULAR FUNCTION AND MASS IN PATIENTS WITH ESSENTIAL HYPERTENTION
- Statin Therapy Can Prevent In-Stent Restenosis (ISR) after Coronary Stenting in the Patient with Acute Coronary Syndrome (ACS)
- STUDIES ON THE SOMATIC AND MENTAL DEVELOPMENT IN EXPERIMENTALLY INDUCED SFD RAT AFTER BIRTH
- MECHANISM OF RETARDED MENTAL DEVELOPMENT IN MSP-INDUCED SFD RATS, ESPECIALLY ON DNA SYNTHETIC RATE OF THE BRAIN
- Upregulation of P-Glycoprotein Activity in Porcine Oocytes and Granulosa Cells During In Vitro Maturation
- Renal biopsy is a helpful tool for the diagnosis of POEMS syndrome
- Development of a Single Bovine Embryo Improved by Co-culture with Trophoblastic Vesicles in Vitamin-supplemented Medium
- Stringent Complete Remission of Primary Plasma Cell Leukemia with Reduced-dose Bortezomib, Lenalidomide and Dexamethasone: A Case Report and Review of the Literature
- Development of a Single Bovine Embryo Improved by Co-culture with Trophoblastic Vesicles in Vitamin-supplemented Medium
- Expression and Regulation of Foxa2 in the Rat Uterus during Early Pregnancy