マウス脾リンパ系細胞幼若化反応におけるマイクロプレ-ト法の基礎的実験条件の検討とその応用

概要

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Experimental conditions for a micromethod to test in vitro mouse spleen cell blastogenic response to Phytohemagglutinin (PHA) and Concanavalin A (Con A) was investigated. Mouse spleen cells revealed the maximum response to 20μg/ml PHA and 10μg/ml Con A on day 3 in culture in RPMI 1640 containing 10 % fetal-calf serum. Mouse spleen cell blastogenic activity, thus determined, increased in accordance with aging, until 25 weeks of age tested. In tumor-bearing mice, spleen cells exhibited the maximum response to PHA and Con A, 6 days and 8 days after tumor inoculation, respectively, and then decreased in accordance with the progress of tumor growth.<BR>Mixed lymphocyte-tumor cell culture reaction (MLTR) was studied using the micromethod condition. The maximum response of spleen cells to Mitomycin C-treated syngeneic tumor cells was observed on the 5th day of culture, when the spleen cells were mixed-cultured with tumor cells in a ratio of 1 : 1. The micromethod described in this paper was show to be one characterized by highsensitivity and reproducibility.
学校法人昭和大学・昭和医学会の論文