Monoamine Oxidaseに関する研究-33-動物血清中のMAO活性測定法の知見補遺

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Monoamine Oxidase (MAO) in serums of beef, cat, pig, dog, rabbit, rat and human were studied by the method of McEwen with a minor improvement and the following results were obtained. MAO activities in beef, cat, pig, dog, rabbit and human serums were found to increase lineally in proportion to increase of serum in reaction mixture. However, MAO in rat serum was not detected with this method. It was also found that the activity units (u) of MAO in serum of human, dog, rabbit, pig, cat and beef were 30, 213, 555, 585, 1110 and 7950 u (m1a mole benzaldehyde product/3 hr/0.6 ml serum), respectively. The optimum pH of rabbit serum MAO was found at pH 8.0. Serum MAO activity was inhibited completely with addition of IIH at the concentration of 1×10-6M and NaCN at the concentration of 1×10-3M. When rabbit serum was incubated at 50°C for 10 min and at 60°C for 10 min, MAO activities were decreased to 92% and 50% of the control value, respectively. The incubation of rabbit serum at 70°C for 10 min resulted in complete inactivation of MAO.
学校法人昭和大学・昭和医学会の論文