Monoamine Oxidaseに関する研究-40-Benzylamine法を用いた微量組織のMAO活性の測定法

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Monoamine oxidase (MAO) activities in serum, kidney, brain and liver from rabbit, dog, rat and mouse were assayed with benzylamine as substrate using McEwen's assay procedure. The original McEwen's method was modified to fit for measurement of MAO activities not only in serum but also in the other various tissues. The modification was accomplished by using a large tube with 2.5 cm of diameter for reaction tube and by shaking it with 100 strokes per minute during the reaction. By this new procedure, it became possible to compare the MAO activity in serum with those in the other tissues. For example, it was found that MAO activities in kideny, brain and liver from rabbit were 101, 21 and 259 times, respectively, of that in serum. While, the MAO activities in serum from rat and mouse were not detected by this method. It became possible to measure MAO activity in the extreme small amount of liver homogenate, such as 0.3 mg of rabbit liver. MAO activities in serum, brain, kidney and liver from rabbit were inhibited completely by pheniprazine (1×10-5 M), but that in serum was not inhibited by pargyline (1×10-5 M) whereas those in kidney, brain and liver were inhibited. Moreover, MAO in serum was inhibited by harmaline (1×10-5 M) whereas those in brain, kidney and liver were not. KCN (10-2M) inhibited MAO in serum and kidney but not inhibited those in brain and liver. These results are suggesting that there are multiple forms of MAO in organs.
学校法人昭和大学・昭和医学会の論文

Monoamine Oxidaseに関する研究-40-Benzylamine法を用いた微量組織のMAO活性の測定法

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