ラット大腸運動に対するモルヒネの影響--in vivoとin vitroでの比較

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Morphine is thought to inhibit gastrointestinal transit by the modulation of neural control and/or direct action to the gut. Our previous studies suggest that morphine inhibits colon motility through the activation of the sympathetic nervous system. The aim of this study was to determine if morphine-induced inhibition on colonic motility is due to either direct action on the colon or the modulation of the autonomic nervous system. Male adult Wistar rats (300-350 g) were anesthetized under a mixture of urethane and α-chloralose. To measure colon motility, a catheter with a balloon sensor was inserted into the colon 5-6 cm from the anus for the in vivo study. Morphine (10 mg/kg) hydrochloride or saline (1 ml/kg) was injected (i.v.) to the experiment group and to the control group, respectively. The longitudinal segment dissected from the colon 5 cm from the anus was suspended in Krebs solution to measure the motility for the in vitro study. As a result, intravenous administration of morphine significantly depressed colonic motility in vivo compared to the control group. The inhibitory effect lasted for about 4 hours. In contrast, colonic motility in vitro restarted after dissection and did not show any significant inhibition. The direct administration of morphine (750μg/ml) and naloxone (100μg/ml) in Krebs solution did not affect colonic motility in vitro. These results suggest that the mechanism of the inhibitory effect of morphine (i.v.) on colonic motility involves indirect action, possibly of the central or autonomic nervous system and might be a result of activation of the sympathetic nervous system.
学校法人昭和大学・昭和医学会の論文

ラット大腸運動に対するモルヒネの影響--in vivoとin vitroでの比較

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