The Biosynthesis of Folic Acid Compounds in Plants:V. Reaction Mechanism of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings

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概要

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• Dihydropteroate synthetase purified from pea seedlings catalyzed the formation of dihydropteroic acid from 2-amino-4-hydroxy-6-hydroxymethyldi-hydropteridine and p-aminobenzoic acid in the presence of ATP and Mg2+. The reaction was stimulated by preincubation of the enzyme with hydroxymethyldihydropteridine, ATP and Mg2+. AT32P was incorporated into the enzyme frac tion by incubation with ATP-γ-32P, hydroxymethyldihydropteridine and Mg2+. Dihydropteroic acid was formed and pyrophosphate was liberated by the incubation with p-aminobenzoic acid from the AT32P-incorporated enzyme solution. AMP was detected as the other product in the reaction.The proposed systematic name of dihydropteroate synthetase is discussed.

• 日本ビタミン学会の論文

著者

• 岩井 和夫

  Research Institute for Food Science, Kyoto University

• 沖中 靖

  Research Institute for Food Science, Kyoto University

• 岩井 和夫

  Research Institute for Food Science Kyoto University

関連論文

• PURIFICATION AND PROPERTIES OF THE DIHYDROFOLATE SYNTHETASE FROM PEA SEEDLINGS
• PURIFICATION AND PROPERTIES OF THE DIHY DROFOLATE SYNTHETASE FROM SERRATIA INDICA
• THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
• Nutritional requirements for folate compounds and some enzyme activities involved in the folate biosynthesis.
• Some characteristics of the dihydrofolate synthetase from Serratia indica.
• Purification and properties of quinolinate phosphoribosyltransferase from the "shitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:V. Reaction Mechanism of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:IV. Purification and Properties of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• THE OCCURRENCE AND PROPERTIES OF METHYLENE-TETRAHYDROFOLATE DEHYDROGENASE IN PEA SEEDLINGS, AND INTRACELLULAR DISTRIBU-TION OF SOME FOLATE-LINKED ENZYMES IN THE PLANT
• The Biosynthesis of Folic Acid Compounds in Plants:I. Enzymatic Formation of Dihydropteroic Acid and Dihydrofolic Acid from 2-Amino-4-hydroxy-6-substituted Pteridines by Cell-free Extracts of Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:III. Distribution of the Dihydropteroate-synthesizing Enzyme in Plants
• Characteristics of quinolinate phosphoribosyltransferase from the "shiitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:II. Some Properties of Dihydropteroate-synthesizing Enzyme in Pea Seedlings

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