Characteristics of quinolinate phosphoribosyltransferase from the "shiitake" mushroom (Lentinus edodes).

スポンサーリンク

概要

• 論文の詳細を見る

• A highly purified preparation of quinolinate phosphoribosyltransferase was obtained from the "Shiitake" mushroom and some characteristics of this enzyme were investigated. Maximum activity was observed at an incubation temperature of 50°C. Monovalent cations generally had no effect, but the divalent and trivalent cations were more or less inhibitory. Fe2+, Ni2+, Zn2+, Al3+ and Fe3+ strongly inhibited the reaction and Cl- and NO3- were also inhibitory. The PRPP saturation curve of this enzyme was sigmoidal. PRPP may be an allosteric effector to this enzyme. Quinolinic acid analogues (pyridine di- and mono-carboxylic acids) exerted no influence on the reaction. Some nucleotides were inhibitory, especially ATP, which was the most potent inhibitor. SH reagents and albocycline did not affect the activity. Possible regulations of the de novo biosynthesis of NAD in the mushroom are discussed.

• 財団法人 学会誌刊行センターの論文

著者

• 田口 寛

  Research Institute for Food Science, Kyoto University

• 岩井 和夫

  Research Institute for Food Science Kyoto University

関連論文

• PURIFICATION AND PROPERTIES OF THE DIHYDROFOLATE SYNTHETASE FROM PEA SEEDLINGS
• PURIFICATION AND PROPERTIES OF THE DIHY DROFOLATE SYNTHETASE FROM SERRATIA INDICA
• THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
• Nutritional requirements for folate compounds and some enzyme activities involved in the folate biosynthesis.
• Some characteristics of the dihydrofolate synthetase from Serratia indica.
• Purification and properties of quinolinate phosphoribosyltransferase from the "shitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:V. Reaction Mechanism of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:IV. Purification and Properties of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• THE OCCURRENCE AND PROPERTIES OF METHYLENE-TETRAHYDROFOLATE DEHYDROGENASE IN PEA SEEDLINGS, AND INTRACELLULAR DISTRIBU-TION OF SOME FOLATE-LINKED ENZYMES IN THE PLANT
• The Biosynthesis of Folic Acid Compounds in Plants:I. Enzymatic Formation of Dihydropteroic Acid and Dihydrofolic Acid from 2-Amino-4-hydroxy-6-substituted Pteridines by Cell-free Extracts of Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:III. Distribution of the Dihydropteroate-synthesizing Enzyme in Plants
• Characteristics of quinolinate phosphoribosyltransferase from the "shiitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:II. Some Properties of Dihydropteroate-synthesizing Enzyme in Pea Seedlings

もっと見る 閉じる

スポンサーリンク