The Biosynthesis of Folic Acid Compounds in Plants:IV. Purification and Properties of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings

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概要

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• Dihydropteroate synthetase was purified from extracts of one day-old pea seedlings by chromatographic methods on DEAE-cellulose, DEAE-Sephadex A-50 and Sephadex G-200. The final enzyme preparation was homogeneous in chromatographic and ultracentrifugal analyses. The purified enzyme utilized 2-amino-4-hydroxy-6-hydroxy-methyldihydropteridine as substrate in the presence of both ATP and Mg2+, as did its pyrophosphate ester in the presence of Mg2+, to produce dihydropteroic acid by coupling with p-aminobenzoic acid. The Km value for pyrophosphorylmethyldihydropteridine was 1.14×10-5M. The enzyme was specific for the amino group at the para-position in benzoic acid, since the reaction was not influenced by the addition of ortho- or meta-aminobenzoic acid. The Km value for p-aminobenzoic acid was 1.11×10-6M.Enzymatic formation of dihydropteroic acid was competitively inhibited by several sulfonamides. Inhibition levels of the various sulfonamides corresponded relatively with the growth inhibition levels by these compounds for Escherichia coli.

• 日本ビタミン学会の論文

著者

• 沖中 靖

  Research Institute for Food Science, Kyoto University

• 岩井 和夫

  Research Institute for Food Science Kyoto University

関連論文

• PURIFICATION AND PROPERTIES OF THE DIHYDROFOLATE SYNTHETASE FROM PEA SEEDLINGS
• PURIFICATION AND PROPERTIES OF THE DIHY DROFOLATE SYNTHETASE FROM SERRATIA INDICA
• THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
• Nutritional requirements for folate compounds and some enzyme activities involved in the folate biosynthesis.
• Some characteristics of the dihydrofolate synthetase from Serratia indica.
• Purification and properties of quinolinate phosphoribosyltransferase from the "shitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:V. Reaction Mechanism of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:IV. Purification and Properties of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• THE OCCURRENCE AND PROPERTIES OF METHYLENE-TETRAHYDROFOLATE DEHYDROGENASE IN PEA SEEDLINGS, AND INTRACELLULAR DISTRIBU-TION OF SOME FOLATE-LINKED ENZYMES IN THE PLANT
• The Biosynthesis of Folic Acid Compounds in Plants:I. Enzymatic Formation of Dihydropteroic Acid and Dihydrofolic Acid from 2-Amino-4-hydroxy-6-substituted Pteridines by Cell-free Extracts of Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:III. Distribution of the Dihydropteroate-synthesizing Enzyme in Plants
• Characteristics of quinolinate phosphoribosyltransferase from the "shiitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:II. Some Properties of Dihydropteroate-synthesizing Enzyme in Pea Seedlings

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