THE OCCURRENCE AND PROPERTIES OF METHYLENE-TETRAHYDROFOLATE DEHYDROGENASE IN PEA SEEDLINGS, AND INTRACELLULAR DISTRIBU-TION OF SOME FOLATE-LINKED ENZYMES IN THE PLANT

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概要

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• Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) which is responsible for the enzymatic convertion of 5, 10-methylene-H4FA to 5, 10-methenyl-H4FA was found in various plant tissues. The enzyme was partially purified from pea seedlings and some of its properties were investigated. The enzyme was purified about 40-fold by fractionation with ammonium sulfate and gel-filtration. The optimum pH was 7.8. 5, 10-Methylene-H4FA and NADP were specifically required in the enzymatic reaction. Their Km values were 3.5×10-4M and 5.1×10-5M, respectively. Intracellular distribution of some folate-linked enzymes, i.e., methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase, and phosphoribosylaminoimidazolecarboxamide formyltransferase, in pea seedlings were investigated. Major portions of these enzymes were located in the soluble fraction, while some activities of these enzymes were significantly detected in mitochondrial or microsomal fraction. A possible pathway for the synthesis and metabolism of folate coenzymes in plants is discussed.

• 財団法人 学会誌刊行センターの論文

著者

• 鈴木 襄

  Shizuoka Eiwa Junior College

• 岩井 和夫

  Research Institute for Food Science Kyoto University

関連論文

• An S-Adenosyl-L-methionine : Cytisine Methyltransferase in Thermopsis Seedlings
• PURIFICATION AND PROPERTIES OF THE DIHYDROFOLATE SYNTHETASE FROM PEA SEEDLINGS
• PURIFICATION AND PROPERTIES OF THE DIHY DROFOLATE SYNTHETASE FROM SERRATIA INDICA
• THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
• Nutritional requirements for folate compounds and some enzyme activities involved in the folate biosynthesis.
• Some characteristics of the dihydrofolate synthetase from Serratia indica.
• Purification and properties of quinolinate phosphoribosyltransferase from the "shitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:V. Reaction Mechanism of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:IV. Purification and Properties of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• THE OCCURRENCE AND PROPERTIES OF METHYLENE-TETRAHYDROFOLATE DEHYDROGENASE IN PEA SEEDLINGS, AND INTRACELLULAR DISTRIBU-TION OF SOME FOLATE-LINKED ENZYMES IN THE PLANT
• The Biosynthesis of Folic Acid Compounds in Plants:I. Enzymatic Formation of Dihydropteroic Acid and Dihydrofolic Acid from 2-Amino-4-hydroxy-6-substituted Pteridines by Cell-free Extracts of Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:III. Distribution of the Dihydropteroate-synthesizing Enzyme in Plants
• Characteristics of quinolinate phosphoribosyltransferase from the "shiitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:II. Some Properties of Dihydropteroate-synthesizing Enzyme in Pea Seedlings

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