The Biosynthesis of Folic Acid Compounds in Plants:I. Enzymatic Formation of Dihydropteroic Acid and Dihydrofolic Acid from 2-Amino-4-hydroxy-6-substituted Pteridines by Cell-free Extracts of Pea Seedlings

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概要

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• Cell-free extracts of pea seedlings contain the enzyme system which catalyzes the formation of folate compounds from 2-amino-4-hydroxy-6-substituted pteridines and p-aminobenzoic acid or p-aminobenzoylglutamic acid. Active pteridines were 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine and its pyrophosphate ester, i.e., 2-amino-4-hydroxy-6-pyrophosphorylmethyldihydropteridine, and a considerable amount of folate compound was formed enzymatically from the latter compound in the presence of Mg2+ as a cofactor, whereas the less amount was formed from the former in the presence of both ATP and Mg2+. The products were characterized, by a bioautographic technique, as dihydropteroic acid and dihydrofolic acid from p-aminobenzoic acid and p-aminobenzoylglutamic acid, respectively, in the presence of pyrophosphorylmethyldihydropteridine. In the enzymatic reaction, p-aminobenzoic acid was more active as substrate than p-aminobenzoylglutamic acid. When p-aminobenzoic acid and L-glutamic acid were used in place of p-aminobenzoic acid in the enzyme system, dihydropteroic acid and dihydrofolic acid were formed in the presence of the pteridine, ATP and Mg2+. From these results presented, the following pathway for the biosynthesis of folate compounds in plants was proposed:

• 日本ビタミン学会の論文

著者

• 沖中 靖

  Research Institute for Food Science, Kyoto University

• 岩井 和夫

  Research Institute for Food Science Kyoto University

• 鈴木 襄

  Research Institute for Food Science, Kyoto University

関連論文

• PURIFICATION AND PROPERTIES OF THE DIHYDROFOLATE SYNTHETASE FROM PEA SEEDLINGS
• PURIFICATION AND PROPERTIES OF THE DIHY DROFOLATE SYNTHETASE FROM SERRATIA INDICA
• THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
• Nutritional requirements for folate compounds and some enzyme activities involved in the folate biosynthesis.
• Some characteristics of the dihydrofolate synthetase from Serratia indica.
• Purification and properties of quinolinate phosphoribosyltransferase from the "shitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:V. Reaction Mechanism of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:IV. Purification and Properties of the Dihydropteroate-synthesizing Enzyme from Pea Seedlings
• THE OCCURRENCE AND PROPERTIES OF METHYLENE-TETRAHYDROFOLATE DEHYDROGENASE IN PEA SEEDLINGS, AND INTRACELLULAR DISTRIBU-TION OF SOME FOLATE-LINKED ENZYMES IN THE PLANT
• The Biosynthesis of Folic Acid Compounds in Plants:I. Enzymatic Formation of Dihydropteroic Acid and Dihydrofolic Acid from 2-Amino-4-hydroxy-6-substituted Pteridines by Cell-free Extracts of Pea Seedlings
• The Biosynthesis of Folic Acid Compounds in Plants:III. Distribution of the Dihydropteroate-synthesizing Enzyme in Plants
• Characteristics of quinolinate phosphoribosyltransferase from the "shiitake" mushroom (Lentinus edodes).
• The Biosynthesis of Folic Acid Compounds in Plants:II. Some Properties of Dihydropteroate-synthesizing Enzyme in Pea Seedlings

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