COLORIMETRIC DETERMINATION AND PHYSICOCHEMICAL PROPERTIES OF HOMOPANTOTHENIC ACID

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概要

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• Through the study of fundamental conditions for colorimetric determination of homopantothenic acid (HOPA) in presence of sodium naphthoquinone sulfonate solution, a standard quantitative analysis and a quantitative separation of HOPA from the decomposition product were established. The physicochemical properties were investigated simultaneously. The general conclusion drawn from the experi ments is as follows.1. The principle in the quantitative determination of calcium homopantothenate was based on the colorimetry of an orange yellow-colored solution obtainable in the reaction of sodium-1, 2-naphthoquinone-4-sulfonate with γ-aminobutyric acid (GABA). It is the alkaline hydrolyzate of HOPA in buffer solutions of pH 8.8-9.6.2. There was no significant variation of analytical values in the use of Clark-Lubs buffer solutions having different pH values between 8.8 and 9.6.3. Colorization was stable and its maximum absorption was present at 465 mμ. The concentrations of HOPA solutions between 200 and 1000μg/25ml were directly proportional to the absorbances.4. The quantitative separation of HOPA from the decomposition product, GABA, was successfully conducted by the removal of GABA by the use of cationicion exchange resine Amberlite CG-120.5. The recovery rate of HOPA by resinous treatment was over 98%.6. The solubility of HOPA in water was approximately 70%, while it was insoluble in organic solvents though the solubility in methanol was 1.3%.7. HOPA was found to be an optically active compound of which specific rotation [α]D20 was+23.8°.8. The apparent dissociation constant, pKa, of HOPA was 4.52 at 25° when calculated from its potentiometric curve.9. One spot was detected on a thin-layer chromatogram using a solvent composed of n-butanol, acetic acid and water.10. Four functional groups, OH, NH, CONH and COO were identified by the infrared spectrophotometry of HOPA.11. The ultraviolet spectrum of HOPA indicated no presence of the peak at the wavelengths over 200mμ.12. Kinetic observation of the stability of HOPA disclosed that it was most stable in pH values ranging from 5 to 6.

• 日本ビタミン学会の論文

著者

• 内海 勇

  Pharmaceutical Research Laboratory Tanabe Seiyaku Co., Ltd.

• 児玉 貞介

  Department of Pediatrics, Wakayama Medical College

• 鮫島 政義

  Pharmaceutical Research Laboratory, Tanabe Seiyaku Co., Ltd.

• 天野 文徳

  Pharmaceutical Research Laboratory, Tanabe Seiyaku Co., Ltd.

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