Selective removal of low molecular substances with monoclonal antibodies.

概要

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After somatic cell fusions between murine spleen cells immunized with several organic anion-protein conjugates and murine myeloma cells, we have prepared many kinds of monoclonal antibodies against haptenic low molecular organic anions. Low molecular organic anions such as metabolic waste, drugs, and toxins usually tightly bind to serum proteins. They therefore cannot easily be removed from blood with various adsorptive resins or hemodialysis. Thus, using these monoclonal antibodies which possesed high affinity enough to compete with serum proteins for the organic anions, we designed a new approach to remove organic anions from blood selectively. As an example, among the monoclonal antibodies raised against bilirubin, we selected one clone which had high affinity enough to compete with serum albumin for bilirubin binding. The monoclonal antibody immobilized on agarose beads showed higher affinity to bilirubin than albumin did, and effectively removed bilirubin in jaundiced human sera in vitro.
一般社団法人日本人工臓器学会の論文