The calcification-promoting action of 1,25(OH)2D3 and calcitonin in rat with 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP=EHDP)-induced rickets - Comparative study of initial site of calcification in the growth plate.:Comparative study of initial site of

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We reported that 1, 25(OH)2D3 (1, 25D) and calcitonin ([Asu1, 7]-eel calcitonin, ECT) promote calcification in the widened growth plate of the HEBP-induced rickets in rats.The experiment lasted 2 weeks and female weanling Wistar King-A rats 4 weeks old were used. 60mg/kg of HEBP was administered subcutaneously daily for 7 days, then the drug was stopped (HEBP rat). 250ng/kg of 1, 25D was given to the HEBP rat for the 14 consecutive days (2), or for the former 7 days (f), or for the latter 7 days (1), that made up 3 groups. 40U/kg of ECT was given in the same 3 ways.In the result, we found that 1, 25D (2) and ECT (2) groups promoted calcification in the growth plate, but when the administration was separated to the former and latter 7 days, only 1, 25D (1) and ECT (f) groups showed enhanced calcification and the rest groups did not.In the present work we observed the spatial extension of the calcification in the growth plate in HEBP rats given 1, 25D or ECT. The growth plate was dissected with a razor, embedded undecalcified, and sectioned in the frontal plane consecutively at a width of 10μ, and the preparation was stained with von Kossa & HE or Safranin-O.In the 1, 25D (1) group, linear calcified zone was in the upper hypertrophic cell zone, while in the ECT (f) group, calcification exists in the lower hypertrophic cell zone.
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