軟体動物の生体防御機構-1-ホタテガイ組織における殺菌素と凝集素の活性

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The extracts of the digestive diverticula (DD) from the normal two-year-old scallop, Patinopecten yessoensis, in hanging cultures were found to possess a low level of natural bactericidal activeity (bactericidin titer: 5 to 10) against HS 29 strain, Arthrobacter sp. No activity, however, was observed in the extracts of the kidney, mantle and gill. The natural agglutinin activity against sheep erythrocytes was extremely high (agglutinin titer: 128) in the DD extracts, as compared with that in the extracts of the gonad, giil and adductor muscle. The natural bactericidal activity of DD which was maximal from March to May declined with the rise of water temperature in the vicinity where scallops were cultivated, and was minimal in hot summer. It exhibited a recovery trend after summer and attained its maximum level in December. The seasonal changes paralleled those in the natural agglutinin activity of DD. The baseline killing power of DD for HS 29 strain was enhanced conspicuously by admi-nistration of HS 29 strain vaccine (formalin-killed, ca. 1×107cells/ml). The duration of the enhancement was approximately 5 days at 10°C.
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