染色体操作法によるアユのクロ-ンの生産とアイソザイムマ-カ-および組織移植法によるその確認〔英文〕

概要

論文の詳細を見る
We attempted to produce clonal fish by chromosome manipulation in ayu Plecoglossus altivelis. To induce mitotic gynogenesis diploids (mitotic-G2N as complete homozygous individuals), the eggs were inseminated with UV-irradiated sperm of normal diploid and were treated with a hy-drostatic pressure of 650kg/cm2 at 80 min after insemination and just before the first cell cleavage. The production of mitotic-G2N was confirmed by isozyme markers. The mitotic-G2N pro-duced from the female parents which were heterozygous at Gpi-1, Me-1 and Pgm loci showed segre-gation into two homozygous types at those loci, and they were female in all individuals. To pro-duce clonal fish, the eggs of mitotic-G2N were inseminated with the UV-irradiated sperm of normal diploid. The diploidization was achieved by retaining the second polar body with cold shock. Two families of clonal fish were produced and designated as clone-1 and clone-2. The clonal fish examined were all homozygous types in which their genotypes were the same as their mothers in each of clonal fish. The graft-tissue (a piece of operculum) transplanted into their intraclonal sibling hosts survived and was accepted. On the other hand, the graft-tissue trans-planted into the different clonal hosts was rejected in both clones. These results suggest that in-traclonal siblings possessed completely identical histocompatibility genes.