ヨーロッパおよびニホンウナギの生化学的識別法に関する研究

概要

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In order to establish a method for identification of the European freshwater eel (Anguilla anguilla L.) and Japanese freshwater eel (Anguilla japonica T. et S.), biochemical characters such as LDH, MDH, esterase and muscle proteins were examined using the starch-gel electrophoretic method. 1) Major zones of LDH emerge at 2.0cm, 3.8cm and 5.1cm from the origin in the European eel, and 2.0cm, 3.7cm and 5.4cm in the Japanese eel in the electrophoresis under the conditions applied. 2) MDH zones emerge at 0.6cm, 1.1cm and 1.6cm from the origin in the European eel, and 1.6cm, 2.9cm and 4.1cm in the Japanese eel. 3) Esterase zones of muscle extract emerge at 5.5cm and 6.7cm in the European eel, and 5.7cm and 6.9cm in the Japanese eel. 4) It is conluded that these electrophoretic characters are available for identification of these two species even in elvers, although a few differences with growth stages and genetic variation are observed in the electropherograms.