クロダイ精子を用いた高水温ショック法によるマダイ雌性発生2倍体の作出〔英文〕

概要

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Gynogenetic diploid red sea bream Pagrus major were produced by fertilizing the eggs with black sea bream Acanthopagrus schlegeli sperm that had been genetically inactivated with ultraviolet (UV) rays, and then 3min. after insemination, heat shocking the eggs at 35°C for 2.5min. to retain the second polar body. This treatment gives a satisfactory survival rate of more than 70% beyond yolk sac resorption. An attempt to produce gynogenetic diploids by suppression of the first cleavage was unsuccessful. There are at least 14 fixed differences at enzyme loci between red and black sea bream that can be detected by electrophoresis. No evidence was found of male contribution in two families of gynogenetic diploids, indicatiing a 100% success rate for induced gynogenesis. In this study, no differences in the growth rates between control and gynogenetic offsprings was observed up to the age of 6 months. However, significant differences in the growth rate between the two families were found either in control or gynogenetic groups. These differences may be caused by maternal effects as well as genetic factors.