IHNV弱毒クロ-ンのIHN防御免疫効果

概要

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The immunogenic activities of attenuated IHNV clones, AF71-1 and AF71-1-CL3, were examined in this study. These two clones had been purified from successively subcultured IHNV by plaquing, subsequently selected through infectivity trials. Rainbow trout and/or kokanee were immunized with the two IHNV clones by intraperitoneal (IP) injection or water-born method, subsequently challenged with virulent IHNVs. By IP injection, the immunization with AF71-1 was extremely effective in rainbow trout and kokanee, but less or no effect by water-born immunization. The immunogenic activity of AF71-1•CL3, which had been clone purified from AF71-1 by limiting dilution method, was determined in rainbow trout and almost the same results as AF71-1 were obtained. Remarkable protective effect was achieved in AF71-1•CL3 immunized fish by IP injection at the amount of 102.0TCID50/fish.