ウナギ肝臓浮遊細胞における糖新生に及ぼすオレイン酸の影響〔英文〕

概要

論文の詳細を見る
Effect of oleic acid on gluconeogenesis in the eel liver was investigated by using the isolated liver cells. Oleic acid at 2.5 and 0.25mM inhibited gluconeogenesis from 5mM lactate by 99 and 36%, respectively. Oleic acid did not inhibit protein synthesis but inhibited the incorporation of 14C-lactate into amino acids-fraction. Glutamate content in the cells was decreased by the addition of 2.5mM oleic acid. Oleic acid and oleoyl-CoA at 10μM inhibited glutamate dehydrogenases of the eel and bovine liver completely. Malate dehydrogenase and lactate dehydrogenase were not inhibited by oleic acid and oleoyl-CoA at 10μM, but oleoyl-CoA stimulated lactate dehydrogenase. 02 consumption and 14CO2 production was slightly increased by the addition of oleic acid.These results suggest that the inhibitory effect of oleic acid on gluconeogenesis is due to the inhibition of glutamate dehydrogenase by oleoyl-CoA. The inhibition of glutamate dehydrogenase leads to the decrease in glutamate content in the mitochondria which inhibits the oxaloacetateaspartate pathway in gluconeogenesis.
日本水産學會の論文