Vibrio anguillarumの病原性と外膜構成成分の変化について〔英文〕

概要

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The plasmidless Vibrio anguillarum strain PT479 obtained after seven passages in ayu Plecoglossus altivelis was highly virulent to ayu. An isogenic strain from the 20th passage in a lab medium had low virulence. The uptake of radioactive iron by the high-virulent strain was more rapid than that of the low-virulent strain. The highly virulent strain was able to grow in an M9 salts medium supplemented with 0.5% (wt/vol) glucose, 20μg each of aspartic acid and histidine per ml, and 2μM transferrin. The low-virulent strain was inhibited under such conditions. The outer membrane protein pattern did not change in either the high-virulent strain or the low-virulent isogenic strain. However, there was a difference in lipopolysaccharide (LPS) structure. A high-molecular weight band was evident in the high-virulent strain. The amount of proteins with the molecular weights ranging from 67, 000 to 100, 000 daltons increased in the outer membrane of both high-and low-virulent strains grown under iron limitation. The production of LPS was enhanced in the high-virulent strain compared to the low-virulent strain as determined by the Limulus test.
社団法人日本水産学会の論文