浸漬ワクチンを行ったアユの血液および腸管内における除菌作用〔英文〕

概要

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Ayu Plecoglossus alaivelis were immersed for 3 min in a 5% NaCl solution containing 1% lyophilized formalin-killed Vibrio anguillarurm. Two months after vaccination, each of the four experimental groups vaccinated fish were infected by intraperitoneal (i.p) injection of either 103, 104, or 106 cells of V. anguillarum PT479 streptomycin resistant (Smr)-strain or exposed to 106 cells/ml of PT479 Smr strain. The time period of elimination of the infecting organism depended on the number of cells of V. anguillarum given to the fish. The challenge organisms wera virtually eliminated within 6h after i.p. injection of 103 or 104 PT479 Smr cells in the blood and intestinal tracts. On the other hand, organisms increased 12h after injection of 103 or 104 cells/ml in non-vaccinated fish, reaching 106 cells/ml of blood and 105 cells/g in the intestinal tracts by 72h. The challenge organisms were observed in the blood and intestinal tracts of vaccinated fish Within 24h after i.p. injection of 106 cells. Subscquentry, the organisms decreased to 10 cells or less. The number of organisms increased in non-vaccinated fish after i.p. injection of 106 cells. The number of challenge organisms was 10 cells/ml or g, or less, in the blood and the intestinal tracts of vaccinated fish 72h after exposure to 106 cells. The challenge organisms were also ob-served in the blood of non-vaccinated fish for 72h.
日本水産學會の論文