コイのα-グロビン遺伝子ファミリ-の解析-1-α-グロビン遺伝子No.3,No.6,およびNo.7の構造解析〔英文〕

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Seven kinds of α-globin genes were isolated from the genomic library of carp Cyprinus carpio chromosomal DNA. In this study, the complete nucleotide sequences of three carp α-globin genes (Nos. 3, 6, and 7) were determined. These carp genes had analogue with the arrangement of trans-lated, untranslated, and intervening sequences of the vertebrate α-globin gene, i.e. two intervening sequences interrupting the coding region into three parts. The coding region of exon 1, 2, and 3 in Nos. 3, 6, and 7 were 95 base pair (bp), 208bp, and 129bp, respectively. The first and second intervening sequences of No. 3 were 159bp and 96bp in length, respectively. The intervening sequences of No. 6 were 179bp and 99bp, and of No. 7 159bp and 92bp in length, respectively. In the 5'- and 3'-untranslated region, sequences homologous with the consensus sequence of the signals for expression were observed. The individuality of the nucleotide sequences suggests that the carp α-globin genes constitute a family structure. The amino acid residues of the predicted carp α-globin concern quaternary structure were extremely conserved with other vertebrate, e.g., human, duck, and frog.
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