Edwardsiella tarda由来のRプラスミド間の系統関係〔英文〕

概要

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The properties and DNA structure of thirteen transferable R plasmids detected in Edwardsiella tarda strains collected in various eel culture ponds were compared. Two R plasmids, encoded for resistance to chloramphenicol (CM), tetracycline (TC) and sulfonamide (SA), inhibited DNA phage growth whereas the remaining R plasmids did not. The restriction and modification systems of these R plasmids were different from those of R plasmids previously reported.1-3) The diges-tion patterns of R plasmid DNAs with HindIII could be divided into four groups. Common sequences were observed in PstI digestions of R plasmids carrying resistance to TC and SA, or CM, TC, and SA. pJA6018 DNA encoded with resistance to TC and SA used as a probe hy-bridized strongly to R plasmids containing the same resistance markers and partially hybridized to R plasmids with resistance to CM, TC, and SA. Aeromonas hydrophila R plasmid encoded with resistance to TC and SA hybridized to the E. tarda R plasmid encoded with the same re-sistance marker. These results suggest that an R plasmid with the identical DNA structure every-where is widely distributed in eel culture ponds in various areas.