病原真菌の微細構造に関する電子顕微鏡的研究 : 第1報超薄切片によるCandida albicansの内部構造について

概要

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By means of ultrathin section and electron microscopic technic the fine structures ofCandida albicansin yeast phase grown on Sabourauds dextrose agar were revealed. Fixation of specimens proved to be superior in revealing the fine structures using a 2 % potassium permanganate aqueous solution (at room temperature for 48 hours) than with an osmic acid solution. Embedding was done in a routine method. The results obtained were as follows: The cell wall, relatively thick, consists of the three layers, two electron less dense ones divided by a dense interspace. The cytoplasmic membrane, very thin, is recognized. The nuclear apparatus is surrounded by a continous double-membraneous layer with pores; The nucleolus-like structures are observed as an electron dense region. The mitochondria with cristae mitochondriales are also surrounded by a continous double-membraneous layer as in the nucleus. The endoplasmic reticulum seems to be connected with the outer layers of both nucleus and mitochondria. The structures resembling the lamella, potassium permanganophilic uniform granules and vacuoles are also observed inside the cytoplasm.
日本細菌学会の論文