薄層および液体クロマトグラフィーによるaflatoxin(B1,B2,G1,G2)の分離,定量

概要

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(1) Thin-layer chromatography: Various kinds of adsorbent and solvent were examined to separate four aflatoxins. They were well separated from each other by development with 5 per cent acetone in chloroform in the case of Silica gel G (ex Merck) as adsorbent, and also with 1_??_3 per cent methanol in chloroform in the case of Silica gel HR (ex Merck). (2) Liquid chromatography: Various kinds of column, for instance alumina, Silica gel and Sephadex G-10, G-25 and LH-20, were examined. Among them, Sephadex G-10 column showed good result for the mutual separation of four aflatoxins. Crude aflatoxins were dissolved in a minimal volume of methanol, and placed on the Sephadex G-10 column, which was developed and eluted with 1 per cent aqueous methanol or with distilled water. The first fraction was a mixture of coloring matters, and then aflatoxins G2, B2, G1, and B1 were successively eluted. Furthermore, the quantitative analysis of aflatoxin over 10 μg was possible by this chromatography within 10 per cent error.
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