貯穀微生物のくん蒸効果に関する研究第5報 Penicillium islandicum (黄変米菌) の胞子,菌糸および米粒内侵入菌糸に対する臭化メチルの殺菌効果

概要

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The present investigation was undertaken to ascertain the difference of fungicidal activity of methyl brmide to the fungal spores settled on the hulled rice and to the mycelia grown on or in the hulled rice. The hulled rice to be inoculated was sterilized at 12 lbs. for 20minutes by autoclaving. Several grades of hyphal growth in the rice grains were obtained by changing the time of incubation. Microscopical obserations were made on hand sections or frozen sections of the rice grains. With regard to the resistance of spores and mycelia to methyl bromide, the latter has less resistance than the former. The mycelia were completely sterilized by fumigation at 45 mg/l, 25℃ for 18hours, but the spores required 50 mg/l for their complete sterilization. The methyl brpmide resistance of the hyphae with in the grain varied according to the extent of mycelial invasion, the resistance of mycelia invaded in the aleuron layer being apparently less than that in the starch layer. The hyphae in the central part of the grain could be sterilized by about the same dosage as in the case of the spores on the grain surface.
日本植物病理学会の論文
1964-01-31