白絹病菌の生化学的研究 : (第2報)有機酸代謝について (2)

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The metabolism of organic acid by Corticium centrifugum was studied in relation to the formation of oxalic acid, and the following results were obtained. 1) Acetate, citrate, succinate, fumarate and malate which are intermediates of the TCA-cycle were well consumed with the fungus mycelium, and the formation of considerable amounts of oxalic acid was found. Whereas little formation of oxalic acid from amino acids such as glutamate, aspartate, and alanine was found. The addition of 10-3M of monoiodo acetate into the medium resulted in the considerable accumulation of oxalic acid. 2) The inhibition effect of malonate on the oxidation of succinate and acetate was not found, but from the facts that various hexoses, glucose-1-phosphate, d-ribose, amino acids and intermediates of the TCA-cycle were well oxidized and from the results of inhibition experiments, the formation of oxalic acid through the TCA-cycle in Corticium centrifugum was suggested. 3) As the oxidation of glucose, gluconic acid and ribose was found, it was suggested that the WARBURG-DICKENs schema in respiration was active in Corticium centrifugum. However, the oxidation of glucose was considerably inhibited with sodium fluoride, and this fact might therefore suggest that the EMBDEN-MEYERHOFs schema was also active. As the oxidation of glucose was considerably inhibited by sodium fluorid, it was suggested that the metalo enzyme such as cytochrome oxidase was responsible as a terminal oxidase.
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白絹病菌の生化学的研究 : (第2報)有機酸代謝について (2)

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