Normal skin and identification of S phase cell in endothelical dermatosis.

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We studied the cells in S-phase in various benign and malignant skin diseases such as psoriasis, seborrheic keratosis, actinic keratosis, Bowen's disease, extramammary Paget's disease, basal cell carcinoma, squamous cell carcinoma, malignant lymphoma, and malignant melanoma. Small pieces of excised skin tumor were incubated with 5-bromo-2'-deoxyuridine (BrdUrd), thymidine analogue, under three atmospheres of pressures in 95% O2 with 5% CO2. Anti-BrdUrd monoclonal antibody stained the nuclei which incorporated that substance. We analyzed labeling pattern as well as the counting of labeling indices. Malignant diseases such as squamous cell carcinoma, basal cell carcinoma, and Bowen's disease showed higher labeling indices than non-malignant specimens. As for the labeling pattern, malignant diseases lost the polarity of labeling and labeled cells were scattered randomly without forming germinative layers. On the other hand, non-malignant specimens showed the germinative layers. Therefore, high labeling indices together with the loss of polarity seemed characteristic for the malignancy.
日本皮膚悪性腫瘍学会の論文