Clinical significance of mitochondrial GOT in serum of patients with liver diseases

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There are two types of isoenzymes of GOT, one in the cytoplasmic fraction of cells (supernatant GOT, s-GOT) and the other in the mitochondria (mitochondrial GOT, -GOT).Wada et al. devised a new method for the direct determination of serum m-GOT. The principle is as follows: s-GOT in the serum is absorbed with sheep red cells sensitized with antibody corresponding to s-GOT and the remaining m-GOT activity in the serum is determined. The authors determined the serum m-GOT from 10 cases of normal subjects and 83 cases of patients with liver diseases by this method. In some cases of acute hepatitis, though the total GOT in serum elevated to 1500 I.U., m-GOT activity showed less than 30 I.U.. However, in one case of fulminant hepatitis, serum total GOT and m-GOT elevated markedly to 3690 I.U. and 295 I.U., respectively. It was recognized that the occurrence of the extensive degeneration and necrosis of liver tissue resulted in the marked increase of serum m-GOT.From these results, it was elucidated that values of m-GOT in the serum of patients with liver diseases were useful to judge the prognosis and had an important significance on the diagnosis of fulminant hepatitis.
財団法人日本消化器病学会の論文