“Hybrid Exercise” Prevents Muscle Atrophy in Association with a Distinct Gene Expression Pattern
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概要
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“Hybrid exercise” utilizing combined electrical stimulation and voluntary muscle contraction has been developed as a muscle exercise method. Although our previous studies have confirmed the effectiveness of the procedure, the mechanisms of its efficacy still remain unclear. In the present study, we identified genes that are specifically expressed in disused muscles, using the semitendinosus muscle from patients who underwent anterior cruciate ligament (ACL) reconstruction. Preoperative exercise was performed by four ACL-injured patients, who were subjected either to hybrid exercise (n=2), electrical stimulation (n=1), or no electrical stimulation (n=1), in addition to standard weight training for 4 weeks. Cross-sectional area (CSA) of the semitendinosus muscle was measured before and after the exercise by magnetic resonance imaging (MRI). A piece of the semitendinosus muscle was isolated during the surgery, and comprehensive analysis of the gene expression in this sample was performed using DNA microarray analysis. CSA increased in size by 4.2 and 14.7%, respectively, after hybrid exercise, and by 1.4% after electrical stimulation. However it shrunk by 7.7% without electrical stimulation. DNA microarray analysis revealed that hybrid exercise was more effective at stimulating the expression of signal transduction-, transcription- and cytoskeleton-related genes in semitendinosus muscles than electrical stimulation alone. In particular, gene ontology analysis revealed that hybrid exercise induced significantly higher expression of eukaryotic translation initiation factor 5A (EIFSA), peroxisomal biogenesis factor 6 (PEX6) and histone cluster 1 H4 (HIST1H4), compared with electrical stimulation alone. The expression of signal transduction-, transcription- and cytoskeleton-related genes may play an important role in muscle bulk increasing mechanisms in hybrid exercise.
著者
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Kohno Shohei
Department Of Neurosurgery Ehime University Graduate School Of Medicine
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Nikawa Takeshi
Department Of Nutrition School Of Medicine The University Of Tokushima
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Nagata Kensei
Department Of Orthopaedic Surgery Kurume University
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Soejima Takashi
Department Of Bacteriology Faculty Of Medical Sciences Kyushu University
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Hirasaka Katsuya
Department Of Nutrition The University Of Tokushima Faculty Of Medicine
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MATSUGAKI Toru
Department of Orthopedic Surgery, Kurume University School of Medicine
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Okumura Yuushi
Department Of Health And Sports Sciences Graduate School Of Frontier Biosciences Osaka University
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Yoshimitsu Kazuhiro
Department Of Orthopedic Surgery Kurume University
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Kohno Shohei
Department of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School
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Hirasaka Katsuya
Department of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School
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SHIBA NAOTO
Department of Orthopaedic Surgery, Kurume University School of Medicine
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MATSUGAKI TORU
Department of Orthopedics, Kurume University School of Medicine
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ISHIDOH KAZUMI
Institute for Health Sciences, Tokushima-Bunri University
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NAGATA KENSEI
Department of Orthopaedic Surgery ,Kurume University School of Medicine
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ISHIDOH Kazumi
Institute for Health Sciences, Tokushima Bunri University
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YOSHIMITSU KAZUHIRO
Department of Orthopedics, Kurume University School of Medicine
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OKUMURA YUUSHI
Department of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School
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