リン脂質の薄層クロマトグラムに対する吸着剤中の微量成分の影響

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1) Authors found that lecithin region appears as two spots (upper spot; Lec (U), lower spot; Lec (L)), on sillica gel plate which developed in a mixture of n-butanol : acetic acid : water (60 : 20 : 20). 2) It was suggested that these chromatographic patterns of lecithin region were caused by some minor contaminations contained in adsorbents. 3) White crystalline substance was obtained from silica gel adsorbent as a major component of extraction with chloroform : methanol : acetic acid (2 : 1 : 1), and it was identified as sodium acetate. 4) As a result, it was found that sodium silicate, sodium carbonate and sodium bicarbonate had similar effects on the silica gel plates for lecithin, but sodium chloride, sodium sulfate, calcium chloride and calcium sulfate had no effect. 5) Sphingomyelin and lyso-lecithin also appeared as two spots such as lecithin on silica gel plates containing sodium acetate with the above developing solvent. Moreover, lyso-lecithin of ten appeared as two spots on silica gel plates containing sodium acetate developed with a mixture of diisobutylketone : acetic acid : water (40 : 25 : 5).
社団法人日本油化学会の論文