アルミナロッド・TLC-FID法での脂質分析

概要

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Methods for quantitatively estimating the composition of simple lipid and phospholipid mixtures were developed using alumina thin-layer chromatography-flame ionization detection systems.<BR>Simple lipid mixtures containing palmitic acid, cholesterol, tripalmitin and cholesterol palmitate, were separated on Chromarod A (alumina sintered rod) with double development to a distance of 6 and 10cm from the start, using benzene-ethylacetate (9 : 1) and <I>n</I>-hexane-benzene (9 : 1). A phospholipid mixture containing phosphatidylcholine, lysophosphatidylcholine, sphingomyeline, cardiolipin and phosphatidylethanolamine were separated on the rod employed with chloroform-methanol-water-15N ammonia solution-pyridine (65 : 27.5 : 4 : 2 : 2). Individual lipids separated on the rod were then automatically quantified by means of a hydrogen flame ionization detecter (Iatroscan).<BR>The relative responses of these lipids were found to be in proportion to the theoretical responses based on weight per cent. These responses converged with a maximum error of 510% (C.V.).
社団法人日本油化学会の論文