シンクログラフィーの脂質分子種定量分析への応用

概要

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Neutral lipids and phospholipids were conveniently separated based on their degree of unsaturation by employing the chromatography on silica gel sintered rod impregnated with 12.5% silver nitrate. Individual lipid species separated on the rod were then quantified <I>in situ</I> with a flame ionization detector (thinchrography). Triglycerides were separated into the individual molecular species using benzene-diethylether (98 : 2, vol/vol) or benzene-chloroform-acetic acid (90 : 10 : 1, vol/vol) as the developing solvent system. The detector responses of tripalmitin, triolein and trilinolein on thinchrography were found to be proportional to the theoretical values based on weight percent. The method has been successfully employed to the analysis of eleven kinds of oils and fats. Each sample was shown to yield a characteristic chromatogram, indicating that this method might be useful for identifying the source of the oils or fats.<BR>Phosphatidylcholine and phosphatidylethanolamine were also separated into the individual molecular species by the same technique with chloroform-methanol-water (65 : 25 : 3, vol/vol) as the developing solvent system.
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