TLC-FID法によるリノール酸メチル及び油脂酸化生成物の定量

概要

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A simple quantitative method for the analysis of oxidized methyl linoleate and edible oils by TLC-FID has been developed. Oxidized methyl linoleate 10200 μg was developed on a Chro marod SII, first by n-hexane to 10 cm and then by n-hexane : diethylether, 8 : 2 vol/vol to 7 cm. This double developing method was found applicable to the quantitation of oxidation products from methyl linoleate. Moreover, the most suitable conditions for quatitating very small amounts of as many oxidized products as possible were determined. A 200μg sample (almost the maximum amount) was spotted on a Chromarod and developed first by η-hexane to 8 cm, then to 10 cm and finally by η-hexane : diethylether 9 : 1 vol/vol to 7 cm. By this triple developing method, 1% of the oxidation products containing in methyl linoleate were quantitated. Both methods are applicable to the analysis of oxidation products in edible oils such as corn and safflower oil.