ステロイドホルモンと血清タンパクとの結合及びタンパク-ホルモン結合に与える脂肪酸の影響

概要

論文の詳細を見る
The binding of steroidhormones to bovine serum proteins was examined so as to explain the transport behavior of steroidhormones by proteins in serum. The affinities of bovine serum proteins prepared by Cohns separation method with six kinds of steroidhormones (α-estradiol, ethynylestradiol, progesterone, androsterone, dehydroisoandrosterone and testosterone) were measured from partition experimental results. The affinity of Cohns fraction III with hormones was not observed, but binding to fraction IV-1 and V (albumin) was clearly evident. When these hormones were added exogenously, binding coefficients K (protein-binding form/free form) in 3.5% bovine serum albumin (BSA) solution were 4080% of those noted in bovine serum containing the same amount of albumin. The effect of the binding of free fatty acid (FFA : C14 : 0, C16 : 0, C18 : 0, C18 : 1 and C18 : 2) with BSA on the affinity of BSA to steroidhormones was also investigated. Change in affinity could not be clearly detected for α-estradiol, ethynylestradiol or dehydroisoandrosterone, but a distinct increase was observed for progesterone, androsterone and testosterone following the addition of FFA. The binding coefficients of the latter three hormones increased by 5060% beyond that of the repeated control without the addition of FFA.
社団法人日本油化学会の論文