ブタノール, エチレングリコール系溶剤を用いたリン脂質のロ紙クロマトグラフィー

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Paper chromatography of phospholipid mixture was carried out with n-butanol, ethylenglycol, water (4 : 1 : 3) and resulted to separate into each constituent. With ascending method for 6 hours at 21°C the mixture of cardiolipin, lecithin and lysolecithin was separated into three spots and Rf values were 0.72±0.02 for cardiolipin, 0.85±0.03 for lecithin and 0.64±0.02 for lysolecithin. The mixture of phosphatidyl ethanolamine and phosphatidyl serine was also separated into two spots, and Rf values at 23°C were 0.80±0.02 for the former and 0.64±0.02 for the latter with same condition as described above. The increase of water content in the solvent system decreased the Rf values of phospholipids, indicating 0.92, 0.84, 0.72±0.02 for cardiolipin and 0.95, 0.90, 0.85±0.03 for lecithin, with solvent systems which were composed of butanol : ethylenglycol : water at the ratio of 4 : 1 : 1, 4 : 1 : 2 and 4 : 1 : 3 respectively.
社団法人日本油化学会の論文