アルキルベンゼンスルフォネート-タンパク質複合体の血清学的研究 : I.アルキルベンゼンスルフォネート-アルブミン複合体

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Alkylbenzensulfonate (ABS)-egg albumin (EA)- and alkylbenzensulfonate-bovine serum albumin (BSA)-complexes (ABS:EA, ABS:BSA) were prepared by mixing each component dissolved in water at pH 7.0-7.5, respectively. Two sorts of ABS, tetrapropylene type and normal alkylene type were used. Each complex comprising 10 mg of protein in the ratio of ABS/protein = 3 was twice injected in rabbits according to Freund's adjuvant method followed by three subcutaneous injections with 1 mg of protein in the ratio of ABS/protein = 3. According to the ordinary precipitin reaction, we found 1 positive reactions in ABS-anti ABS:EA, and ABS:EA-anti ABS:EA systems, And we also saw 2 positive reactions in the case of BSA-anti ABS:BSA, 2' or 1 positive reactions in that of ABS:BSA-anti ABS:BSA, respectively. But ABS solution concentrated more than 0.04% of ABS showed 1 or 1' positive reactions with all rabbit sera. Titers of antibody were 1:1 in EA-anti ABS:EA system against 1:256 or 1:512 in that of EA-anti EA, and 1:4 or 1:8 in the case of BSA-anti ABS:BSA against 1:16 or 1:32 with BSA-anti BSA. Arthus phenomenon showed 2 positive reaction for BSA: anti ABS:BSA system against 4 positive reaction with BSA-anti BSA system.
一般社団法人日本アレルギー学会の論文
1966-10-25