ラット灌流肝臓をもちいた環境中化学物質による細胞内代謝動態変化の観察方法 : 灌流肝臓における呼吸鎖チトクローム類, チトクロームP450, カタラーゼの可視域スペクトル変化, および還元型ピリジンヌクレオチドの螢光変化の経時的同時測定

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An experimental system was developed to observe the dynamic effects of environmental chemical agents on the intracellular functions associated with the reduction-oxidation transitions. This system consisted of the non-recirculating rat liver perfusion with hemoglobin-free buffer, the surface fluorometry, the scanning reflectance spectrophotometry, and the combined oxygen electrodes. The real-time, continuous and simultaneous measurements of the reducing equivalents, the spectral changes of cytochrome aa3, b and c, cytochrome P-450, and catalase, and the oxygen uptake by the liver were carried out in the perfused rat liver. Typical changes following the administration of nitrogen, carbon monoxide, potassium cyanide, hexobarbital, metyrapone, and/or ethanol were demonstrated. Due to its simplicity, the system can be potentially useful in assessments of environmental chemical agent effects, alone and in combination, on the intracellular dynamic function, and in screenings.