重金属の Paramecium tetraurelia に対する影響 : I 細胞分裂に及ぼす影響

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The effects of heavy metal compounds (MnCl2⋅Mn(CH3COO)2⋅PbCl2⋅Pb(CH3COO)2⋅CdCl2⋅Cd (CH3COO)2⋅HgCl2⋅K2Cr2O7) on cell division in Paramecium tetraurelia were investigated. Cells used in the present experiments had previously induced autogamy; they were vigorous and divided about 8 times in 40 hours at 25°C. In a cell population that had been reproduced from a cell isolated two days before, nine cells (control group) were isolated and placed in normal medium and groups of six cells (experimental groups) were isolated and placed in media that contained heavy metals at different concentrations. Cultures were then incubated at 25°C for two days, then the number of cell divisions was counted.The number of cell divisions was gradually reduced at concentrations of manganese more than 100μM, of lead more than 30μM, of cadmium more than 2μM, of mercury more than 2μM, and of chromium (VI) more than 10μM. These concentrations of Pb, Cr (VI) and Cd are nearly the same to as the standard concentrations allowed in drainage in Japan. This suggests that the cell of paramecium can be used to test the toxicity of heavy metals.
日本衛生学会の論文