条虫免疫に関する研究-2-

概要

論文の詳細を見る
Hemagglutination tests as described by Boyden were conducted for the detection of antibodies against Sparganum mansoni antigen.Human blood cells were sensitized with tannic acid and coated with the anigen prepared from dried Sparganum mansoni worm by sonic disintegration.Antisera were obtained from the rabbit immunized by intravenous injection of the antigen.The following results were botained.1. It was shown that antibodies in the immune serum were successfully titrated by the hemagglutination test.2. The optimum concentration of tannic acid for the treatment of red cells was 1:50, 000 dilution. Red cells treated with 1:20, 000 dilution of tannic acid showed often non-specific agglutination. The reactions were negative, when red cells treated with 1:100, 000 dilution of tannic acid were used.3. It was observed that 1:3, 000 dilution of the dried antigen was the adequate concentration to sensitize tannic acid cells, and the highest dilution of the antiserum for positive reaction was 1:1, 600.
日本衛生学会の論文