A New Radioimmunoassay Method for Serum Triiodothyronine using Sephadex G25 Column
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概要
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A new method for the measurement of triiodothyronine (T<SUB>3</SUB>) in sera was described. All through the assay, a small column of Sephadex G25 suspended in 0.1N NaOH was employed. After the preparation of the column in 2.5m<I>l</I> syringe, 250μ<I>l</I> 0.1N NaOH solution containing <SUP>125</SUP>I-T<SUB>3</SUB>, 100μ<I>l</I> standard solution and 100μ<I>l</I> T<SUB>3</SUB>-free serum or sample serum were added and passed through the column. After washing out with 2.0m<I>l</I> of 0.063 M phosphate buffer pH7.4, 0.4m<I>l</I> diluted antiserum (1: 300) was added. The column was counted by a well counter (the first count). After 1hr incubation, the column was washed again with 1.5m<I>l</I> phosphate buffer. The second count of the column representing unbound T<SUB>3</SUB> radioactivity was performed. Percent retention was calculated from the ratio of the second to the first count. Percent retention was shown to relate to the dose of T<SUB>3</SUB> in the range from 31.3 to 1, 000pg/100μ<I>l</I>.<BR>The concentration of T<SUB>3</SUB> in sera of 12 normal, 4 TBG-deficient, 9 hypothyroid, and 13 hyperthyroid subjects was 148.3±24.4, 52.5±20.7, 53.3±27.2 and 425.4±110.8 ng/100m<I>l</I> (mean±SD), respectively.
- 社団法人 日本内分泌学会の論文
著者
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SHISHIBA YOSHIMASA
Division of Endocrinology and Endocrine Research Laboratory Toranomon Hospital and Okinaka Memorial
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SHIMIZU TAEKO
Division of Endocrinology and Endocrine Research Laboratory, Toranomon Hospital and Okinaka Memorial
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YOSHIMURA SHIZUKO
Division of Endocrinology, Toranomon Hospital
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SHIMIZU TAEKO
Division of Endocrinology and Endocrine Research Laboratory, Toranomon Hospital
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SHIMIZU TAEKO
Division of Endocrinology, Toranomon Hospital
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YOSHIMURA SHIZUKO
Division of Endocrinology and Endocrine Research Laboratory, Toranomon Hospital
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