Inactivation of Human Thyroid Stimulator by Human Thyroid Extract

概要

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To determine whether the thyroid stimulating activity of IgG of patients with Graves disease is associated with the reaction with a putative human thyroid antigen, the inactivation of the property of IgG to stimulate cAMP generation in human thyroid slices incubated <I>in vitro</I> was studied by pretreating the IgG with human thyroidal particulate fraction. In the preliminary experiment, it was demonstrated that to cause cAMP generation stimulation, on incubation period of120min is required to allow the IgG to penetrate the tissue. When human thyroid slices were incubated with normal IgG without or with pretreatment by human thyroid particulate fraction obtained from 100mg tissue, cAMP content in the slices was 142±25 or 138±26 f moles/mg, respectively, indicating that basal thyroidal cAMP levels were not influenced at all by normal IgG even after pretreatment with thyroid particulate fraction. When the slices were incubated with IgG of Graves disease without or with the similar pretreatment, cAMP content was 320±31 or 140±25f moles/mg, respectively, demonstrating an almost complete inhibition of the activity of the IgG to cause cAMP generation stimulation.
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