Extraction and Partial Purification of Prolactin-Release Stimulating Factor in Bovine Hypothalami
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概要
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Partial purification of prolactin-release stimulating factor (PRF) was performed by Sephadex G-25 gel filtration of bovine hypothalamic extracts. PRF activity was evaluated on the basis of the measurement of immunoreactive prolactin released from the isolated rat hemipituitary <I>in vitro</I>. PRF activity was found in the fractions with Kav=0-0.49 and prolactin-release inhibiting activity was also detected in the fractions with =0.69-0.89. The dose-response relationship was established between the partially purified PRF and its activity. The elution position of the partially purified PRF preceded that of TRH on Sephadex G-25. TRH at the dose of 100nM stimulated the release of TSH <I>in vitro</I>, but not the release of prolactin.<BR>These results may indicate that there exists PRF with a relatively high mo lecular weight in the bovine hypothalamus.
- 社団法人 日本内分泌学会の論文
著者
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YAMASAKI MAKOTO
Department of Surgery, Graduate School of Medicine, Osaka University
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SAWANO SHINJI
Department of Endocrinology and Metabolism, Toranomon Hospital
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SHIRAKI Masaru
Research Institute for Polymers and Textiles
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KOKUBU TOMOKUNI
Research Institute for Polymers and Textiles
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ISHIZUKA YUSAKU
Department of Chemistry, College of General Education, University of Tokyo
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Yamasaki Makoto
Department Of Safety Assessment Research Laboratories Roussel Morishita Co. Ltd.
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Sawano Shinji
Department Of Endocrinology And Metabolism Toranomon Hospital
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YAMASAKI MAKOTO
Department of Chemistry, College of General Education, University of Tokyo
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YAMASAKI MAKOTO
Department of Chemistry, College of General Education, Uniuersity of Tokyo
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